The CustomArray system is a unique system for the in situ synthesis of oligonucleotide microarrays made to your specifications. You design the the content for your microarrays, or you can use the design services that we offer. We ship the microarrays to you within 30 business days.
The custom oligonucleotide microarray is synthesized using a semiconductor-based electrochemical-synthesis process. Each oligonucleotide probe is synthesized via a platinum electrode that is independently controlled by the synthesizer’s computer. Synthesis is based on established phosphoramidite chemistry and occurs at thousands of sites simultaneously according to a computer algorithm that activates only specified electrodes. Since physical photolithographic masks or pre-built collections of oligos are not involved in the process, all probes can be easily changed without extra time or cost.
The oligonucleotides are synthesized in a proprietary porous reaction layer that is over the surface of the semiconductor. The resultant chip is shown below
Building a diverse CRISPR gRNA library using arrayed-synthesized oligo pools. Application note
Advantages of using array-synthesized, double stranded DNA capture probes in NGS targeted sequencing Application note
Assembly of precise mutant libraries using arrayed-synthesized oligos for protein optimization Application note
CustomArray's chips use electrochemical detritylation to control DNA synthesis. Electrochemical detritylation can be a much-more-efficient method to synthesize oligonucleotides than light-based synthesis processes. This means the oligonucleotides are of highest quality, and the sensitivity of the microarray is maximized. Since physical photolithographic masks or pre-built collections of oligos are NOT involved in the process, all probes can be easily changed without extra time or costs.
This depends on a number of characteristics. Most pools ship with at least 1 ug of total DNA in solution.
The turnaround time of your pool will be stated on the quote you receive. Most orders ship within 9-12 business days.
Yes, but we recommend keeping the length difference of the oligos within 10%. If larger variations are necessary, please contact CustomArray.
CustomArray has an in situ synthesis technology. Oligonucleotide probes are synthesized using the chip. This means there are no upfront costs of buying hundreds of different oligonucleotides, maintaining inventory, and spotting these agents. Making changes to chip designs and layouts is rapid and easy.