The CustomArray system is a unique system for the in situ synthesis of oligonucleotide microarrays made to your specifications. You design the the content for your microarrays, or you can use the design services that we offer. We ship the microarrays to you within 30 business days.

The custom oligonucleotide microarray is synthesized using a semiconductor-based electrochemical-synthesis process. Each oligonucleotide probe is synthesized via a platinum electrode that is independently controlled by the synthesizer’s computer. Synthesis is based on established phosphoramidite chemistry and occurs at thousands of sites simultaneously according to a computer algorithm that activates only specified electrodes. Since physical photolithographic masks or pre-built collections of oligos are not involved in the process, all probes can be easily changed without extra time or cost.

The oligonucleotides are synthesized in a proprietary porous reaction layer that is over the surface of the semiconductor. The resultant chip is shown below

Building a diverse CRISPR gRNA library using arrayed-synthesized oligo pools. Application note

Advantages of using array-synthesized, double stranded DNA capture probes in NGS targeted sequencing Application note

Assembly of precise mutant libraries using arrayed-synthesized oligos for protein optimization Application note

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Welcome to the Frequently Asked Questions (FAQ page).
Below, we have tried to answer the most common questions about our products and services. If you find that your question is not answered on this page, please email us at:
info@customarrayinc.com

HOW IS CUSTOMARRAY'S TECHNOLOGY DIFFERENT FROM OTHER IN SITU SYNTHESIS TECHNOLOGIES?

CustomArray's chips use electrochemical detritylation to control DNA synthesis. Electrochemical detritylation can be a much-more-efficient method to synthesize oligonucleotides than light-based synthesis processes. This means the oligonucleotides are of highest quality, and the sensitivity of the microarray is maximized. Since physical photolithographic masks or pre-built collections of oligos are NOT involved in the process, all probes can be easily changed without extra time or costs.

HOW MUCH PRODUCT WILL I RECEIVE?

This depends on a number of characteristics. Most pools ship with at least 1 ug of total DNA in solution.

HOW LONG BEFORE I RECEIVE MY OLIGO POOL?

The turnaround time of your pool will be stated on the quote you receive. Most orders ship within 9-12 business days.

CAN I HAVE OLIGOS OF DIFFERENT LENGTHS IN A POOL?

Yes, but we recommend keeping the length difference of the oligos within 10%. If larger variations are necessary, please contact CustomArray.

DOES AN OLIGO POOL NEED TO BE AMPLIFIED PRIOR TO USE AND DO YOU OFFER THAT SERVICE?

It is highly recommended to amplify a pool prior to use as it overcomes a number of technical challenges. For more information contact CustomArray.
Some protocols such as Ligation-independent Cloning do not require amplification

HOW TO DESIGN AN OLIGO POOL SEQUENCE?

Our oligo pool length range from 10-170 nt. As most application need the oligo pool to be amplified, we recommend to add universal 5’ and 3’ primer sequence to each oligo. You may add restriction enzyme site if you need to remove the priming sequence after amplification.
We also recommend avoid poly sequence and keep the length difference within 10%.

HOW ARE CUSTOMARRAY MICROARRAYS DIFFERENT FROM SPOTTED ARRAYS?

CustomArray has an in situ synthesis technology. Oligonucleotide probes are synthesized using the chip. This means there are no upfront costs of buying hundreds of different oligonucleotides, maintaining inventory, and spotting these agents. Making changes to chip designs and layouts is rapid and easy.

WHAT CAN I USE CUSTOMARRAY'S MICROARRAYS FOR?

From CustomArray, you can order whole microarrays just like ordering oligos!
The arrays can be used for for applications including gene expression, miRNA expression analysis, rapid identification of pathogens in biological samples, identification of prevalent flu strains and flu type within patient samples, comparative genome hybridization (CGH), SNP analysis and detection, re-sequencing, and chromatin immunoprecipitation (ChIP) on chip. Optimized probes can be designed based against a set of genes submitted by a customer as GenBank accession numbers or raw sequence data. We can also accept customer-specified oligonucleotide probe sequences.