bar

MiRNA EXPRESSION ANALYSIS

Species-Specific MicroRNA arrays

The microRNA product suite is built on CustomArray's 4X2K format. The catalog Species-Specific MicroRNA arrays consist of Human, Mouse, Rat, and Arabidopsis microarrays built with probes from the miRBase (formerly Sanger Database). We can use the latest version of the database to build your content.

Species-Specific MicroRNAs are listed below. To obtain the probe list of any of the MicroRNA's or to order a microarray contact us at info@customarrayinc.com.

Species Specific
MicroRNA
Genus Species

MicroRNA Probes*

Controls
Product Number
hands

Human

Homo sapiens
964
32
1000071-7802
mouseimg

Mouse

Mus musculus
647
33
1000071-7803
ratimg

Rat

Rattus norvegicus
685
33
1000071-7804
arabidopsisimg

Arabidopsis

Arabidopsis thaliana
950
32
1000071-7801

Species-Specific MicroRNA Information:

  • Designs are based on unique microRNA species from current release of miRBase (Sanger database) (http://www.mirbase.org/)
  • Hairpin sequences are excluded from the design process
  • Probes are anti-sense to mature microRNA
  • For each probe, a double mismatch control is included to ensure specificity of detection. These ‘2mut’ controls are designed to maximally de-stabilize probe-target hybridization, thus, mutations are introduced in the two most stable triplets.
  • Non-microRNA controls include positive controls corresponding to small RNA such as tRNA and U6. The small RNA co-isolate with microRNA if the target preparation protocol is based on size fractionation.
  • The negative controls contain anti-sense probes designed for abundant mRNA (such as Actb (actin), HSPA4, PGK1). If target samples have some RNA degradation, the partially digested fragments of the abundant mRNA will co-isolate with microRNA during size fractionation, and the corresponding probes will produce high intensity signals. If target samples are of good quality, the negative control signals will stay low.
  • MicroRNA Microarrays are compatible with common reagents and fluorescent array scanners
  • All probes on every microRNA are 100% quality tested
  • Rapid turn-around time

Content Choice MicroRNA built by CustomArray

Users can select the subset of organisms from the miRBase for the Content Choice MicroRNA offered by CustomArray. Users can define the content as well as the controls on the MicroRNA array.

Also, for microRNA profiling or other microRNA services, or to learn more about any of our products or services, please contact us at info@customarrayinc.com.